Spider Gwen
In this reality the radioactive spider bit Gwen Stacy rather than Peter Parker giving her the abilities of a spider. She goes by the name Spider-Woman and much like Spider-Man of Earth 616 used her newly found powers to for more personal gain rather than helping people by becoming a professional drummer. Peter Parker remained a weak individual that Gwen needed to save from bullying. This made him develop an inferiority complex causing him to create a serum to make himself more like Gwen after she has revealed to him her powers. This causes him to become Earth 65's version of the Lizard and Gwen is forced to fight him. Unfortunately due to the serum during the battle Peter dies and she vows to use her powers for good. Unfortunately Gwen is blamed for Peter's death and a campaign against her (spearheaded by J. Jonah Jameson) is started.

Gwen is the drummer of a band named the Mary Janes (Which includes Mary Jane Watson, Glory Grant and an unknown third member) although she is often late for practices and concerts. While out fighting crime she is also targeted by the police force who is being commanded by her own father Captain George Stacy. Due to Captain Stacy's success against organised crime he a hit is placed on him by the Kingpin who uses his lawyer Matt Murdock to hire hit man Aleksei Sytsevich (who in the regular continuity is the villainous Rhino). Aleksei tracks Captain Stacy down at a Mary Janes concert and attacks him there, Gwen steps in and is able to defeat him but is cornered by her own father. There she reveals to him that she is Spider-Woman and he allows her to escape.

Gwen is recruited to take part in the Spider-Verse event where Spider-Men from every reality are being hunted down by Morlun and his family. She is one of Spider-Man's (616) right hand members but he is unwilling to risk her in dangerous situations since he couldn't save his own Gwen Stacy. They promise to look after each others backs. On one mission Gwen travels to a reality that after this reality's Gwen Stacy died Spider-Man became bitter and more ruthless, eventually turning into the Hobgoblin. Gwen tries to recruit him and even gets through to him but they are attacked by members of Morlun's family. Peter sacrifices himself to save Gwen and with his last words he assures her that he is dying a happy man and that she in truth had saved him.

This version of Gwen Stacy first appeared in Edge of Spider-Verse issue 2 and has been well received by fans and has been the subject of numerous pieces of fanart and cosplay. Her popularity even convinced Marvel to give Gwen her own solo series based after the events of Spider-Verse. She is currently depowered because an encounter with Earth-65 Cindy Moon and relies on "power-ups" given to her by her world's Kingpin (Matthew Murdock).